Specific binding of phorbol ester tumor promoters
نویسندگان
چکیده
منابع مشابه
Specific binding of phorbol ester tumor promoters to intact primary epidermal cells from Sencar mice.
The binding of [20-3H]phorbol 12,13-dibutyrate ([3H]PDB) to intact living epidermal cells in monolayer culture was characterized. At 37 degrees C, the maximum specific [3H]PDB binding (binding displaceable by 30 microM unlabeled PDB) was attained in 15--20 min and was followed by a rapid decrease (down regulation) of radioactivity bound to the cells. The activity lost by the cells during this d...
متن کاملEffect of Phorbol Ester Tumor Promoters on the Expression of Melanogenesis in B-16 Melanoma Cells1
Cells of the C3 clone of B-16 melanoma synthesize melanin only at confluence after which they senesce and can no longer be passaged. Addition to the cultures of 10~8-10~7 M 12-Otetradecanoylphorbol-13-acetate (TPA) shortly after plating de layed by about 2 days the onset of melanogenesis. TPA did not, however, affect the growth of the cells or the time at which they reached confluence. The abil...
متن کاملEffects of phorbol ester tumor promoters in platelet aggregation and platelet production of cyclooxygenase products.
The tumor promoters, 12-O-tetradecanoylphorbol-i 3-ace tate (TPA), phorbol-i 2,13-didecanoate, and mezenein, in the range of 10_8 Mcaused aggregation of washed rabbit platelets. The lag time between the addition of tumor promoters and the onset of aggregation was inversely proportional to the concen tration of the compounds. 4a-Phorbol-i 2, i 3-didecanoate, which is inactive as a tumor promoter...
متن کاملEffect of phorbol ester tumor promoters on the expression of melanogenesis in B-16 melanoma cells.
Cells of the C3 clone of B-16 melanoma synthesize melanin only at confluence after which they senesce and can no longer be passaged. Addition to the cultures of 10(-8)--10(-7) M 12-O-tetradecanoylphorbol-13-acetate (TPA) shortly after plating delayed by about 2 days the onset of melanogenesis. TPA did not, however, affect the growth of the cells or the time at which they reached confluence. The...
متن کاملEffects of Phorbol Ester Tumor Promoters in Platelet Aggregation and Platelet Production of Cyclooxygenase Products1
The tumor promoters, 12-O-tetradecanoylphorbol-i 3-ace tate (TPA), phorbol-i 2,13-didecanoate, and mezenein, in the range of 10_8 Mcaused aggregation of washed rabbit platelets. The lag time between the addition of tumor promoters and the onset of aggregation was inversely proportional to the concen tration of the compounds. 4a-Phorbol-i 2, i 3-didecanoate, which is inactive as a tumor promoter...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1980
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.77.1.567